av3 cell line  (European Collection of Authenticated Cell Cultures)

Journal: Environmental science and pollution research international

Article Title: The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranes.

doi: 10.1007/s11356-025-36137-5

Figure Lengend Snippet: Fig. 1 The effect of DINCH and MINCH on the toxicity of AV3 cells was assessed by measuring LDH release in a culture medium. AV3 cells were exposed for 24 h or 48 h with PPARγ agonist rosiglitazone (AGO) at 1 μM, DINCH (D) at concentration of 0.1, 1, or 10 μM or MINCH (Mi) at 2.5, 10, or 100 μM, either alone or in combination with the agonist (A1D or A1Mi). Results for each exposure condition were compared to 0.2% DMSO con- trol. Each condition was tested in duplicate, and the experiment was conducted four times

Article Snippet: The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for 24 h or 48 h in DMEMF12-GlutaMAXTM (21,041,025, GibcoTM, Fisher Scientific, Illkirch-Graffenstaden, France).

Techniques: Concentration Assay

Journal: Environmental science and pollution research international

Article Title: The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranes.

doi: 10.1007/s11356-025-36137-5

Figure Lengend Snippet: Fig. 2 The effect of DINCH and MINCH on the viability of AV3 cells was evaluated through formazan release measurement. The cells were exposed in the same manner as previously described. For each condition tested, the results were com- pared to 0.2% DMSO control. Each condition was performed in duplicate, and the experiment was repeated four times

Article Snippet: The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for 24 h or 48 h in DMEMF12-GlutaMAXTM (21,041,025, GibcoTM, Fisher Scientific, Illkirch-Graffenstaden, France).

Techniques: Control

Journal: Environmental science and pollution research international

Article Title: The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranes.

doi: 10.1007/s11356-025-36137-5

Figure Lengend Snippet: Fig. 4 A Representative Western blot of PPARγ-protein extracted from AV3 cells after 24 h of treatment. AV3 cells were treated in the same manner as previously described. B The effect of DINCH and MINCH on PPARγ protein expression. The cells were exposed in the same manner as previously described. For each condition tested, the results were com- pared to 0.2% DMSO control. Each condition was performed in duplicate and the experiment was repeated four times

Article Snippet: The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for 24 h or 48 h in DMEMF12-GlutaMAXTM (21,041,025, GibcoTM, Fisher Scientific, Illkirch-Graffenstaden, France).

Techniques: Western Blot, Expressing, Control

Journal: Environmental science and pollution research international

Article Title: The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranes.

doi: 10.1007/s11356-025-36137-5

Figure Lengend Snippet: Fig. 5 The effect of DINCH and MINCH on PPARγ transcriptional activity. AV3 cells were treated for 24 h with DMSO 0.2% (control), PPARγ agonist rosiglitazone (AGO), PPARγ antagonist GW9662 (ANT) at 1 μM, or in combination (AA), DINCH (D) (0.1, 1, or 10 μM), MINCH (Mi) (2.5, 10, or 100 μM) or in combination with

Article Snippet: The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for 24 h or 48 h in DMEMF12-GlutaMAXTM (21,041,025, GibcoTM, Fisher Scientific, Illkirch-Graffenstaden, France).

Techniques: Activity Assay, Control

Journal: Environmental science and pollution research international

Article Title: The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranes.

doi: 10.1007/s11356-025-36137-5

Figure Lengend Snippet: Fig. 6 The effect of DINCH and MINCH on IL6 and IL8 mRNA expression. Expression of IL6 and IL8 transcripts were evaluated by RT-qPCR. AV3 cells were treated with cytomix and for 24 h or 48 h with DINCH (D) (0.1, 1, or 10 μM), MINCH (Mi) (2.5, 10, or

Article Snippet: The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for 24 h or 48 h in DMEMF12-GlutaMAXTM (21,041,025, GibcoTM, Fisher Scientific, Illkirch-Graffenstaden, France).

Techniques: Expressing, Quantitative RT-PCR

Journal: Environmental science and pollution research international

Article Title: The anti-inflammatory effect of the amniotic PPARγ pathways is not dysregulated by the alternative plasticizer DINCH and its metabolite MINCH in human fetal membranes.

doi: 10.1007/s11356-025-36137-5

Figure Lengend Snippet: Fig. 7 The effect of DINCH and MINCH on IL6 and IL8 protein expression. Release of IL6 and IL8 protein was evaluated using Ella™ assay in culture medium. AV3 cells were treated with cytomix and for 24 h or 48 h with DINCH (D) (0.1, 1, or 10 μM), MINCH

Article Snippet: The AV3 amniotic epithelial cell line (CCL-21, obtained from ATCC) was cultured under standard conditions (37 °C, 5% CO2, 95% humidified air) for 24 h or 48 h in DMEMF12-GlutaMAXTM (21,041,025, GibcoTM, Fisher Scientific, Illkirch-Graffenstaden, France).

Techniques: Expressing

Journal: mSphere

Article Title: Inhibition of multiple staphylococcal growth states by a small molecule that disrupts membrane fluidity and voltage

doi: 10.1128/msphere.00772-23

Figure Lengend Snippet: Treatment with D66 reduced the number of viable S. aureus broth HG001 persister cells and modestly decreased the load of USA300 in mammalian cells. (AD) Overnight cultures of the indicated strains were treated at time 0 with DMSO, ciprofloxacin (4 µg/mL), vancomycin (10 µg/mL), or four different concentrations of D66. Cultures were plated for enumeration of CFU at the time points indicated. Mean and SEM of three biological replicates are shown. ( E ) RAW 264.7 macrophage-like cells (blue) and HeLa cells (red) were infected with S. aureus USA300, at multiplicities of infection (MOIs) of 0.5 and 2.5, respectively. Cells were treated 2 hours after infection with DMSO (circles on y axis), vancomycin (vanc, 50 µg/mL, upside-down triangles), daptomycin (dapto, 50 µg/mL, right-side up triangles), or dilutions of D66 from 40 µM (stars for RAW264.7 cells, squares for HeLa cells). After 8 hours of infection, cells were lysed and plated for enumeration of CFU. Mean and SEM of biological duplicates performed with technical triplicates are shown. Non-linear regression; four-parameter variable slope. The IC 50 values are indicated.

Article Snippet: For experiments performed with HeLa cells (ATCC CCL-2), 1 × 10 4 cells were seeded per well.

Techniques: Infection